Targeted genome editing in tetraploid potato through transient TALEN expression by Agrobacterium infection.
Identifieur interne : 000018 ( Main/Exploration ); précédent : 000017; suivant : 000019Targeted genome editing in tetraploid potato through transient TALEN expression by Agrobacterium infection.
Auteurs : Shuhei Yasumoto [Japon] ; Satoru Sawai [Japon] ; Hyoung Jae Lee [Japon] ; Masaharu Mizutani [Japon] ; Kazuki Saito [Japon] ; Naoyuki Umemoto [Japon] ; Toshiya Muranaka [Japon]Source :
- Plant biotechnology (Tokyo, Japan) [ 1342-4580 ] ; 2020.
Abstract
Genome editing using site-specific nucleases, such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat-CRISPR-associated protein 9 (CRISPR-Cas9), is a powerful technology for crop breeding. For plant genome editing, the genome-editing reagents are usually expressed in plant cells from stably integrated transgenes within the genome. This requires crossing processes to remove foreign nucleotides from the genome to generate null segregants. However, in highly heterozygous plants such as potato, the progeny lines have different agronomic traits from the parent cultivar and do not necessarily become elite lines. Agrobacteria can transfer exogenous genes on T-DNA into plant cells. This has been used both to transform plants stably and to express the genes transiently in plant cells. Here, we infected potato, with Agrobacterium tumefaciens harboring TALEN-expression vector targeting sterol side chain reductase 2 (SSR2) gene and regenerated shoots without selection. We obtained regenerated lines with disrupted-SSR2 gene and without transgene of the TALEN gene, revealing that their disruption should be caused by transient gene expression. The strategy using transient gene expression by Agrobacterium that we call Agrobacterial mutagenesis, developed here should accelerate the use of genome-editing technology to modify heterozygous plant genomes.
DOI: 10.5511/plantbiotechnology.20.0525a
PubMed: 32821228
PubMed Central: PMC7434673
Affiliations:
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Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045</wicri:regionArea><wicri:noRegion>Kanagawa 230-0045</wicri:noRegion></affiliation></author><author><name sortKey="Umemoto, Naoyuki" sort="Umemoto, Naoyuki" uniqKey="Umemoto N" first="Naoyuki" last="Umemoto">Naoyuki Umemoto</name><affiliation wicri:level="1"><nlm:affiliation>RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045</wicri:regionArea><wicri:noRegion>Kanagawa 230-0045</wicri:noRegion></affiliation></author><author><name sortKey="Muranaka, Toshiya" sort="Muranaka, Toshiya" uniqKey="Muranaka T" first="Toshiya" last="Muranaka">Toshiya Muranaka</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871</wicri:regionArea><wicri:noRegion>Osaka 565-0871</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant biotechnology (Tokyo, Japan)</title><idno type="ISSN">1342-4580</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genome editing using site-specific nucleases, such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat-CRISPR-associated protein 9 (CRISPR-Cas9), is a powerful technology for crop breeding. For plant genome editing, the genome-editing reagents are usually expressed in plant cells from stably integrated transgenes within the genome. This requires crossing processes to remove foreign nucleotides from the genome to generate null segregants. However, in highly heterozygous plants such as potato, the progeny lines have different agronomic traits from the parent cultivar and do not necessarily become elite lines. <i>Agrobacteria</i> can transfer exogenous genes on T-DNA into plant cells. This has been used both to transform plants stably and to express the genes transiently in plant cells. Here, we infected potato, with <i>Agrobacterium tumefaciens</i> harboring TALEN-expression vector targeting <i>sterol side chain reductase 2</i> (<i>SSR2</i>) gene and regenerated shoots without selection. We obtained regenerated lines with disrupted-<i>SSR2</i> gene and without transgene of the TALEN gene, revealing that their disruption should be caused by transient gene expression. The strategy using transient gene expression by <i>Agrobacterium</i> that we call Agrobacterial mutagenesis, developed here should accelerate the use of genome-editing technology to modify heterozygous plant genomes.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32821228</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1342-4580</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>2</Issue><PubDate><Year>2020</Year><Month>Jun</Month><Day>25</Day></PubDate></JournalIssue><Title>Plant biotechnology (Tokyo, Japan)</Title><ISOAbbreviation>Plant Biotechnol (Tokyo)</ISOAbbreviation></Journal><ArticleTitle>Targeted genome editing in tetraploid potato through transient TALEN expression by <i>Agrobacterium</i> infection.</ArticleTitle><Pagination><MedlinePgn>205-211</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5511/plantbiotechnology.20.0525a</ELocationID><Abstract><AbstractText>Genome editing using site-specific nucleases, such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat-CRISPR-associated protein 9 (CRISPR-Cas9), is a powerful technology for crop breeding. For plant genome editing, the genome-editing reagents are usually expressed in plant cells from stably integrated transgenes within the genome. This requires crossing processes to remove foreign nucleotides from the genome to generate null segregants. However, in highly heterozygous plants such as potato, the progeny lines have different agronomic traits from the parent cultivar and do not necessarily become elite lines. <i>Agrobacteria</i> can transfer exogenous genes on T-DNA into plant cells. This has been used both to transform plants stably and to express the genes transiently in plant cells. Here, we infected potato, with <i>Agrobacterium tumefaciens</i> harboring TALEN-expression vector targeting <i>sterol side chain reductase 2</i> (<i>SSR2</i>) gene and regenerated shoots without selection. We obtained regenerated lines with disrupted-<i>SSR2</i> gene and without transgene of the TALEN gene, revealing that their disruption should be caused by transient gene expression. The strategy using transient gene expression by <i>Agrobacterium</i> that we call Agrobacterial mutagenesis, developed here should accelerate the use of genome-editing technology to modify heterozygous plant genomes.</AbstractText><CopyrightInformation>© 2020 The Japanese Society for Plant Cell and Molecular Biology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yasumoto</LastName><ForeName>Shuhei</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sawai</LastName><ForeName>Satoru</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyoung Jae</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mizutani</LastName><ForeName>Masaharu</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>Kazuki</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Umemoto</LastName><ForeName>Naoyuki</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muranaka</LastName><ForeName>Toshiya</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Biotechnol (Tokyo)</MedlineTA><NlmUniqueID>101233156</NlmUniqueID><ISSNLinking>1342-4580</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">TALEN</Keyword><Keyword MajorTopicYN="N">genome editing</Keyword><Keyword MajorTopicYN="N">potato</Keyword><Keyword MajorTopicYN="N">steroidal glycoalkaloids</Keyword><Keyword MajorTopicYN="N">transient gene expression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32821228</ArticleId><ArticleId IdType="doi">10.5511/plantbiotechnology.20.0525a</ArticleId><ArticleId IdType="pmc">PMC7434673</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Hortic Res. 2018 Mar 02;5:13</Citation><ArticleIdList><ArticleId 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last="Mizutani">Masaharu Mizutani</name><name sortKey="Muranaka, Toshiya" sort="Muranaka, Toshiya" uniqKey="Muranaka T" first="Toshiya" last="Muranaka">Toshiya Muranaka</name><name sortKey="Saito, Kazuki" sort="Saito, Kazuki" uniqKey="Saito K" first="Kazuki" last="Saito">Kazuki Saito</name><name sortKey="Sawai, Satoru" sort="Sawai, Satoru" uniqKey="Sawai S" first="Satoru" last="Sawai">Satoru Sawai</name><name sortKey="Umemoto, Naoyuki" sort="Umemoto, Naoyuki" uniqKey="Umemoto N" first="Naoyuki" last="Umemoto">Naoyuki Umemoto</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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